华南地区蔬菜作物上马铃薯Y病毒属病毒发生情况和种类鉴定

来源 :中国植物病理学会2014年学术年会 | 被引量 : 0次 | 上传用户:uniw0909
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  马铃薯Y病毒属(Potyvirus)是植物病毒第二大属,该属的一些种可侵染多种蔬菜作物并引起严重损失。为了弄清Potyvirus在华南地区蔬菜作物上的发生情况,2011 ~ 2014年,我们对广东、广西等地蔬菜作物病毒病发生与为害情况进行了调查,并采集病毒病疑似病样226份,包括南瓜、节瓜、白瓜、丝瓜、黄瓜、西葫芦和冬瓜等葫芦科蔬菜病样115份,辣椒、番茄、马铃薯等茄科蔬菜病样75份,豆科蔬菜病样9份,十字花科蔬菜病样8份,以及菜田附近种植的烟草16份和百合3份。应用Potyvirus病毒简并引物S[1]/PCR1[2],对疑似病样总RNA进行RT-PCR检测,结果显示,葫芦科、茄科、十字花科和豆科蔬菜作物中Potyvirus阳性率分别为70%、66%、50%和55%,百合和烟草中Potyvirus阳性率为100%和84%。
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