【摘 要】
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It is well established that individual cells differ from each other in many aspects due to stochastic biological processes and differences in environmental perturbations.However,the underlying biochem
【机 构】
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Department of Chemistry,Tsinghua University,Beijing 100084,China;The Key Laboratory of Chemical Biol
【出 处】
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第九届全国微全分析系统学术会议、第四届全国微纳尺度生物分离分析学术会议、2014国际微流控芯片与微纳尺度生物分离分析学术
论文部分内容阅读
It is well established that individual cells differ from each other in many aspects due to stochastic biological processes and differences in environmental perturbations.However,the underlying biochemical characters and mechanisms cannot be revealed by population-level measurement techniques.Microfluidic chip technique has become a useful tool in the single cell isolation and analysis research.In this paper,we represent a high-throughput and label-free single-cell lipids analysis method using microfluidic chip and MALDI-MS.A microwell-based microfluidic chip integrated single cell capture,cell culture and MALDI-MS analysis pretreatment functions was developed.With a capture efficiency of approximately 100%,the acquired single cell arrays were directly detected by MALDI-MS without any sample transfer process.Phospholipids of every single cells in the array were detected by MALDI imaging mass spectrometry(IMS)method to achieve a high-throughput analysis(1-2 cells per second)without any labeling.The established method provides a useful tool for rapid and efficient single-cell metabolites and components analysis.
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