Meloidogyne spp.is a widespread nematode and it can cause serious damage of plants.Secretary proteins synthesized in the esophageal glands and secreted through the stylet of plant parasitic nematodes have critical roles in plant-parasite interactions.Furthermore, the stage of root-knot nematode is highly related to the development of giant cells (GC) which is the pivotal part of the entire pathogenesis.As host giant cells was proved with significant morphological change during the adult stage, comparison of the adult female Meloidogyne incognita secretomes with J2 secretomes at proteomic level and analysis of proteins associated with cell death would be indispensable for exploring the unique unclear pathogenicity of adult female Meloidogyne incognita.This study compared the secretary proteins from J2 larva with the soluble proteins from esophageal gland cells (SPEGC) of adult female Meloidogyne incognita.As a result, 177 unique SPEGC were found successfully in adult female nematode with a wide range of Gene Ontology (GO)categories including death.The evidence for proving adult female root-knot nematode has different parasite-host interaction with J2 larva was preliminarily found initially using bioinformatics at proteomic level.With the GO analysis, 17 proteins associated with death were found in adult female Meloidogyne incognita and signal transduction analysis was further performed.Comparing death-related proteins within different stages of Meloidogyne incognita, 5 unique death-related proteins of adult female were found.Secretome, localization and signaling analysis were performed.Therefore,a new aspect was pointed for the subsequent research about degradation of GC as well as the pathogenesis of adult female Meloidogyne incognita.