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Tetraploid Locust’ is one of the most popular and commercially valuable cultivars.An attempt was performed to explore propagation techniques by softwood cuttings for nursery industry.Results showed that cuttings pretreated with Indole-3-butyric acid (IBA) 1400 mg/L for 2 h soaked method got the best rooting traits (rooting percentage is 77.63%,root number is 8.73 and root length is 3.23 cm).Therefore pre-treatment with IBA 1400 mg/L was recommended and used in further experiments for biochemical research.The adventitious rooting was obtained in three distinct phases i.e.induction (0-15 d),initiation (15-20 d) and expression (20-30 d).Indole-3-acetc acid oxidase (IAAox) activity with IBA treatment increased slightly as compared to the control.IAAox activity increased at induction and expression phases and decreased at initiation phase.Peroxidase (POD) activity with IBA treatment increased from the induction up to the expression phase.Polyphenol Oxidase (PPO) activity increased both in IBA treatment and control at induction phase but declined at initiation and expression phases.Content of Indole-3-acetc acid (IAA) and abscisic acid (ABA) had much different during rooting.IAA content with IBA treatment increased steadily as compared to control.ABA content of the control increased steadily as compared to IBA treatment.IAAox activity and IAA content might be playing vital roles in rooting.