一个新柘橙病毒属病毒全基因组序列测定及分析

来源 :中国植物病理学会第十一届青年学术研讨会 | 被引量 : 0次 | 上传用户:wangxiangbin
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  利用RT-PCR、5-RACE和3-RACE技术测定了薯蓣褪绿坏死花叶病毒(Yam chlorotic necrotic mosaic virus,YCNMV)全基组序列。生物信息学分析表明,该病毒基因组全长为8137nt(不包括Poly(A)尾),基因组编码一个2623个氨基酸长的多聚蛋白。与马铃薯Y病毒科病毒相比,YCNMV 缺少一个P1基因,并且HC-Pro基因相对较短。但是在P3基因编码框中仍存在一个P3-PIPO(Pretty Interesting PotyviridaeORF)编码蛋白。进一步分析结果表明,YCNMV与先前报道的柘橙病毒属病毒——中国薯蓣坏死斑驳病毒(Chinese yam necrotic mosaic virus,CYNMV)全基因组核酸序列同源性最高(同源性为75%)。目前,YCNMV已被第九次国际病毒命名委员会收录为柘橙病毒属一个暂定种。
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