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目的:建立高效液相色谱法测定中药辛夷中木兰脂素含量。方法:采用Zorbaz SB-C_8(4.6mm×150mm,5μm)柱,保护柱C_8(4.6mm×20mm,10μm),以乙腈-四氢呋喃-水(35:1:64)为流动相,流速1mL·min~(-1),检测波长为278 nm,柱温:室温。结果:木兰脂素的线性范围为0.1～1.5μg,r为0.999 9,平均回收率为97.5％,RSD为0.72％(n=5)。结论:该方法准确、简便、快速,灵敏度高,重现性好。为辛夷建立了新的质量控制方法。
Objective: To establish a HPLC method for the determination of magnolidin in Chinese herbs. METHODS: A Zorbaz SB-C_8 (4.6 mm×150 mm, 5 μm) column was used. The guard column C_8 (4.6 mm×20 mm, 10 μm) was used. The mobile phase was acetonitrile-tetrahydrofuran-water (35:1:64). The flow rate was 1 mL·min. ~ (-1), detection wavelength is 278 nm, column temperature: room temperature. Results: The linear range of magnolacin was 0.1-1.5 μg, r was 0.999 9, the average recovery was 97.5%, and the RSD was 0.72% (n=5). Conclusion: The method is accurate, simple, rapid, high sensitivity and good reproducibility. Established a new quality control method for Xinyi.