【摘 要】
：
本文建立了基于毛细管的核酸等温扩增反应(cLAMP)方法，实现了直观、稳定、多重和即时的核酸分析。该方法集成了玻璃毛细管或塑料毛细管，可引入样品和反应试剂液滴，并利用水滴来隔离不同样品和防止污染，通过使用微型加热器提供热量完成反应，并利用手持式手电筒进行荧光信号的可视读出。该系统可实现多个血浆样品中人类免疫缺陷病毒(HIV)的两个靶标RNA的同时检测，对质粒样品的检测灵敏度可达两个拷贝。该核酸检测
【机 构】
：
国家纳米科学中心,北京市海淀区中关村北一条11号,100190 北京大学,北京市海淀区颐和园路5号
论文部分内容阅读
本文建立了基于毛细管的核酸等温扩增反应(cLAMP)方法，实现了直观、稳定、多重和即时的核酸分析。该方法集成了玻璃毛细管或塑料毛细管，可引入样品和反应试剂液滴，并利用水滴来隔离不同样品和防止污染，通过使用微型加热器提供热量完成反应，并利用手持式手电筒进行荧光信号的可视读出。该系统可实现多个血浆样品中人类免疫缺陷病毒(HIV)的两个靶标RNA的同时检测，对质粒样品的检测灵敏度可达两个拷贝。该核酸检测方法完全独立于外部电源和设备，在资源贫乏地区的即时检测方面具有广阔的应用前景。
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