原生质体融合技术构建高效降解棉酚菌株的研究

来源 :第八届全国微生物资源学术暨国家微生物资源平台运行服务研讨会 | 被引量 : 0次 | 上传用户：sinner888

【摘要】

：

【作者】

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赵静陈建军

【机构】

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河南师范大学生命科学学院,河南新乡,453000

【出处】

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第八届全国微生物资源学术暨国家微生物资源平台运行服务研讨会

【发表日期】

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2016年10期

【关键词】

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原生质体融合游离棉酚脱毒发酵

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　　棉酚是存在于棉籽粕中的一种对动物有害的物质，因此若将棉籽粕作为动物饲料蛋白源，其利用率会大大提高(不足30％).本研究在失活的热带假丝酵母原生质体与未失活的酿酒酵母原生质体，在35％的聚乙二醇(PEG6000.A.R)和0.1MCa*诱导下融合，形成融合子.经培养鉴定，失活的热带假丝酵母代谢淀粉的糖化酶系统，在融合子中被未失活的酿酒酵母原生质酶系激活，以淀粉培养基为筛选培养筛选得到融合子.采用间苯三酚法对酿酒酵母，热带假丝酵母，融合菌株进行棉酚降解能力的比较，融合菌株降解棉酚能力显著高于亲本菌株，最终以融合菌株为发酵菌株，棉粕为发酵底物，进行固态发酵.该菌株的获得，能为其在饲料工业化生产上的应用奠定基础.

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原生质体融合技术构建高效降解棉酚菌株的研究

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