Next-generation sequencers and genome editing technologies have played significant role in studying genomics in the field of biology,drug screening,and clinical diagnosis.1,2 To obtain good sequencing results,it is necessary to clearly understand size distributions and concentrations of NGS library.Therefore,quality control(QC)is essential.Previously,agarose electrophoresis and real-time PCR were utilized to confirm size distributions and concentrations of NGS library,respectively.However,the processes involving a series of tedious manual operations are very complicated.Here we report an efficient QC method for NGS library using a fully automated microchip electrophoresis,MultiNA.This method simultaneously obtained size distribution and concentration of NGS library as shown in Figure 1.On the other hand,genome editing using nucleases has become a powerful tool for genomics.In the process of genome editing,there is the possibility of gene knockout due to the error of gene repair,resulting in mutation types.Previously indentifying mutation types is realized by sequencing genome,and it takes lots of cost and time.Here we report an efficient and high-throughput approach for screening targeted mutagenesis by an automated microchip electrophoresis system MultiNA.Screening principle is based on different mutation types have unique band patterns as shown in Figure 2 because of different DNA fragment sizes.