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在基因治疗中,理想的基因转移载体应具备如下的条件:(1)基因转移效率高。(2)容量大。(3)选择性基因转移。(4)受控制的细胞毒性。(5)受调节的免疫原性。(6)易于构建和操作。(7)费用低。(8)安全且易于应用。(9)可被医生及患者接受。 腺病毒(Ad)载体是目前最理想的载体之一,其原因如下:(1)作为真核基因调控模型,Ad载体已被广泛研究并了解了其特性,这是载体开发的坚实基础。(2)Ad载体有中等大小的基因组(平均约36kb),它适合开发容量大而含有最少病毒序列的载体。(3)它们相当稳定,易于获得高滴度(10~(11)～
In gene therapy, ideal gene transfer vectors should have the following conditions: (1) High gene transfer efficiency. (2) Large capacity. (3) Selective gene transfer. (4) Controlled cytotoxicity. (5) Regulated immunogenicity. (6) Easy to build and operate. (7) The cost is low. (8) Safe and easy to apply. (9) Acceptable by doctors and patients. The adenovirus (Ad) vector is one of the most ideal vectors at present. The reasons are as follows: (1) As an eukaryotic gene regulation model, the Ad vector has been extensively studied and understood its characteristics. This is a solid foundation for vector development. (2) The Ad vector has a medium-sized genome (about 36 kb in average) and is suitable for the development of vectors with large capacity and minimal viral sequences. (3) They are quite stable and easily obtain high titer (10~(11)~