Object:effects of GlcN on the embryonic development, level of O-GlcNAc and related gene expression of buffalo embryos were examined in this study.Methods:Buffalo zygotes derived from in vitro fertilization (IVF)were randomly allocated into culture in the medium supplemented with different concentration of GlcN (0, 1, 2, 4 mM) during the different culture period (0-72 h, 72-172 h and 0-172 h).The expression of O-GlcNAc, OGT and OGA in embryos developed from different culture treatment at different developmental staged was determined by IHC and QRT-PCR.