Grass carp as raw materials,electronic nose and solid phase micro-extraction (SPME)combined with gas chromatography-mass spectrometry(GC-MS) to study the way of death effects on the volatile component.The way of death are killing,removed the gill,cryogenic death and natural death.The way of death can effect the lipoxygenase(LOX)s activity.The highest activity of LOX is 450U/per ml crude enzyme solution in the killing grass carp red meat.