【摘 要】
：
为获得贵州特色品种黑山羊与黔北麻羊IL-2基因,本试验以ConA刺激的贵州黑山羊与黔北麻羊外周血淋巴细胞为材料,采用RT-PCR方法分别扩增贵州黑山羊与黔北麻羊IL-2基因,进行克隆与生物信息学分析.结果显示,贵州黑山羊与黔北麻羊IL-2基因核苷酸与氨基酸一致性为100％,完整编码阅读框大小为468bp,编码155个氨基酸.黔北麻羊、贵州黑山羊IL-2基因与Genbank中羊源IL-2基因核苷酸一
【机 构】
：
贵州大学动物科学学院,贵阳,550025 毕节市动物产品质量安全监督检验所,贵州毕节,551700
论文部分内容阅读
为获得贵州特色品种黑山羊与黔北麻羊IL-2基因,本试验以ConA刺激的贵州黑山羊与黔北麻羊外周血淋巴细胞为材料,采用RT-PCR方法分别扩增贵州黑山羊与黔北麻羊IL-2基因,进行克隆与生物信息学分析.结果显示,贵州黑山羊与黔北麻羊IL-2基因核苷酸与氨基酸一致性为100％,完整编码阅读框大小为468bp,编码155个氨基酸.黔北麻羊、贵州黑山羊IL-2基因与Genbank中羊源IL-2基因核苷酸一致性为95.9％～100％,氨基酸一致性为91.1％～100％.遗传进化树结果表明,贵州黑山羊与黔北麻羊IL-2基因处于同一进化分支,与羊源IL-2基因亲缘关系较近.生物信息学分析结果显示,IL-2蛋白二级结构中以α螺旋与无规则卷曲较多,该蛋白为亲水性蛋白,含信号肽序列,无跨膜结构,无特征功能结构域.不同动物IL-2蛋白的抗原性分析存在差异,贵州黑山羊/黔北麻羊IL-2蛋白与羊源IL-2蛋白的比较差异较小,与人和其它动物的比较差异较大.
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