Aim: Nonalcoholic fatty liver disease (NAFLD) is characterized by macrovesicular hepatic steatosis and chronic inflammation, leading to increasing morbidity and mortality.Tribbles homolog 3 (TRB3) was indentified to mediate diabetic cardiomyopathy, As the tightly crosslink between NAFLD and diabetic cardiomyopathy, in this study, we set out to examine the role of TRB3 in preventing NAFLD.