【摘 要】
：
目的 构建人结肠癌裸鼠皮下移植瘤模型、经脾注射肝转移模型及盲肠原位移植瘤模型.方法 将人结肠癌细胞(HCT-116)接种于裸鼠皮下及脾被膜下,分别构建皮下移植瘤模型和经脾注射肝转移模型；以裸鼠皮下肿瘤为瘤源,将其剪切后移植于另一组裸鼠盲肠浆膜下,构建原位移植瘤模型.[结果]皮下移植瘤模型均有成瘤,但未见到局部侵袭、淋巴转移及血行转移等现象；经脾肝转移模型建模6 周后脾脏成瘤率为100％(6/6),
【机 构】
：
首都医科大学附属北京朝阳医院肿瘤科 北京市朝阳区工体南路8号 100020 首都医科附属北京朝阳医
【出 处】
：
第五届广州国际肿瘤学会议暨第一届中国抗癌协会肿瘤靶向治疗专业委员会年会
论文部分内容阅读
目的 构建人结肠癌裸鼠皮下移植瘤模型、经脾注射肝转移模型及盲肠原位移植瘤模型.方法 将人结肠癌细胞(HCT-116)接种于裸鼠皮下及脾被膜下,分别构建皮下移植瘤模型和经脾注射肝转移模型；以裸鼠皮下肿瘤为瘤源,将其剪切后移植于另一组裸鼠盲肠浆膜下,构建原位移植瘤模型.[结果]皮下移植瘤模型均有成瘤,但未见到局部侵袭、淋巴转移及血行转移等现象；经脾肝转移模型建模6 周后脾脏成瘤率为100％(6/6),肝转移率为66.7％(4/6),同时部分裸鼠出现了腹腔转移和腹水形成；盲肠原位移植瘤模型中,12 周后裸鼠盲肠原位成瘤率为80％(4/5),肝转移率为40％(2/5).[结论]皮下移植瘤模型可作为研究实体瘤局部生长的模型；经脾注射肝转移模型肝转移率较高,为研究结肠癌肝转移提供了可靠的模型；原位移植瘤模型最大程度地模拟了在体结肠癌的生物学行为,是较为理想的动物模型.
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