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目的以有效部位为指标,研究葛根芩连片质量控制新方法。方法采用紫外分光光度法(UV)测定总黄酮含量;采用高效液相色谱法(HPLC)测定葛根素含量,菲罗门C18色谱柱(4.6mm×250mm,5μm),流动相为甲醇-水(28:72),流速为1.0ml/min,柱温30°C,检测波长为250nm。结果总黄酮含量为12.26%～12.73%(RSD为1.21%);葛根素含量为2.24%～2.43%(RSD为2.93%)。结论葛根总黄酮含量间变异比葛根素小,更适合作为质量控制指标。
Objective To study the effective method for the quality control of Gegenqinqian contiguous slice with the effective site. Methods The content of total flavonoids was determined by UV spectrophotometry. The content of puerarin was determined by high performance liquid chromatography (HPLC). Phenol C18 column (4.6 mm × 250 mm, 5 μm) was used with the mobile phase of methanol-water 28:72), flow rate 1.0ml / min, column temperature 30 ° C, detection wavelength of 250nm. Results The content of total flavonoids was 12.26% ~ 12.73% (RSD 1.21%). Puerarin content was 2.24% ~ 2.43% (RSD 2.93%). Conclusion The variation of the total flavonoids content of Pueraria lobata is smaller than that of puerarin, which is more suitable as a quality control index.