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目的研究贵州省鼠疫耶尔森菌的生物学特征,探讨疫源地性质。方法对37株鼠疫菌进行生化试验、营养需求试验、毒力基因检测、随机扩增多态性DNA分析和脉冲场电泳分析。结果37株鼠疫菌均不发酵鼠李糖和甘油,发酵麦芽糖和阿拉伯糖,脱氮阳性。选择20株代表菌株检测均为苯丙氨酸依赖(Phe+)、谷氨酸半依赖(Glu±);用Pla、Cafl、inv、hms4对引物分别进行PCR扩增,均得到456、249、1000和700bp的目标基因条带;随机引物(RAPD)分析均获得505、790、1140和1680bp的条带;脉冲场电泳图谱显示338、242.5、168和77kbp的条带。结论贵州省鼠疫菌株的生物学特征与云南省滇闽居民生态型鼠疫菌相同。
Objective To study the biological characteristics of Yersinia pestis in Guizhou Province and explore the nature of the epidemic. Methods Thirty - seven Y. pestis strains were biochemically tested, their nutritional requirements were tested, virulence genes were tested, and random amplified polymorphic DNA analysis and pulsed field electrophoresis were performed. Results None of the 37 Y. pestis strains produced rhamnose and glycerol, fermented maltose and arabinose, and were positive for denitrification. The 20 strains of representative strains were selected for phenylalanine dependent (Phe +) and glutamic acid semi-dependent (Glu ±). The primers were respectively amplified by Pla, Cafl, inv and hms4, And 700bp target gene bands respectively. Random amplified polymorphic DNA (RAPD) analysis showed bands of 505, 790, 1140 and 1680bp. The pulsed-field electrophoresis showed bands of 338, 242.5, 168 and 77kbp. Conclusion The biological characteristics of plague strains in Guizhou Province are similar to those of Y. pestis in Yunnan and Fujian provinces.