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Alzheimers disease(AD)is the most common cause of dementia.As one of the main pathological features,amyloid β(Aβ)deposition is a key pathological hallmark of AD.MicroRNAs (miRNAs)are small non-coding RNAs involved in postranscriptional gene regulation.Significant changes of miRNAs in the brains of AD patients have been revealed in several researches.Meanwhile some reports expounded that miRNAs participated Aβ metabolism and the neuronal loss process.However,a systematic assessment of the miRNA expression profile induced by Aβ-activated signal pathways is still lacking.In the present study,we applied high throughput Illumina sequencing to characterize miRNA expression profiles in the cortexes of APPswe/PS1ΔE9 mice and wild type mice.Using bioinformatics method,we predicted and analysed the biological function of the 45 miRNAs which have significant expression change in statistics and their relation with PI3K/AKT/mTOR signal pathway.We also qualified the relative expression level in APP mice and wildtype mice using qRT-PCR.Additionally,we detected the expression levels of some significant miRNAs in APP mice and wildtype mice at different time points.At last,we made an effort in exploring the role of miR-99b and miR-100 in AD,basing on their trend of firstly up and next down,and found over-expression of miR-99b and miR-100 in PC12 cell lines could lead to apoptosis through downregulating the protein levelof mTOR.