(Research Objective)visfatin, one of the adipocytokines, has close relationship with diseases, like insulin resistance, metabolic syndrome and type Ⅱ diabetes.So we aimed to discuss the effect of aerobic exercise on the expression of visfatin in the tissues of heart, liver and skeleton muscle in obese mice in this study.Research Methods Twenty-four male C57BL/6J mice were randomly divided into 3 groups: normal control group, obesity control group and obesity exercise group(n=8 for each group).Normal control group were fed with normal diet while the obesity groups with high-fat diet.Free feeding and 12 hours illumination every day.After eight weeks feeding, the obesity model were successfully built.Then the obesity exercise group mice were intervened with aerobic treadmill exercise (10 m/min, 1 h/day,, 6 days/week) for eight weeks, and recorded weight every three days.All animals were fasted ovemight and anesthesiaed to collect blood from heart, and collect the tissues from liver, left ventricular and quadriceps femoris muscle after last training.The content of CHO and TG in serum were determined by ELISA; Total protein of lysates were detected by BCA method after extracting from tissues.Then the content of visfatin in each tissue were detected by Western blot analysis, with β-actin as internal reference(β-actin polyclonal antibody 1∶1000, Biovision company), visfatin Polyclonal Antibody as primary antibody (1∶1000,,Biovision company) and IgG(H+G) as second antibody(1∶8000, Beijing ZHong SHan Jin Qiao company).All data were analysized using one-way analysis of variance, withp<0.05 indicating significant differences.Research Results(1)After eight weeksfeeding, weights of obesity control group and obesity exercise group were 26.53±0.29 g and 27.05±1.03 g, both higher 20% than normal control group (21.97±3.46 g) (p<0.05) stating that the model of obesity was built successfully, but there was no significant differences between the two obesity groups.After eight weeks exercise, weights of obesity control group and obesity exercise group changed into 35.37±2.51 g and 31.19±3.5 g,, which were also higher than normal control group (24.73±2.51 g) above 20% (p<0.05).(2) Levels of CHO in serum in normal control group, obesity control group and obesity exercise group were 2.49±0.44 mmol/L, 3.56±0.70 mmol/L and 4.14±0.49 mmol/L.Differences between normal control group and the two obesity groups both were significant (p<0.05), but there was no significant differences between obesity control group and exercise group.Levels of TG in obesity control group and obesity exercise group were 0.58±0.18 mmol/L and 0.50±0.12 mmol/L, both higher than that in normal group (0.37±0.05 mmol/L) (p<0.05), but there was no significant difference between the two obesity groups.(3) Content of visfatin in the tissue of heart in obesity exercise group(0.42±0.04) were higher than that significantly in obesity control group (0.21±0.04) (p<0.05) and normal control group(0.16±0.04) (p<0.01).In the tissue of liver, content of visfatin in obesity exercise group(1.04±0.08) were higher than that in obesity control group(0.33±0.06) (p<0.05) and normal control group(0.29±0.11) (p<0.05).As to quadriceps femoris muscle, visfatin in normal control group, obesity control group and obesity exercise group in turn were 0.47±0.36, 0.41±0.53 and 0.14±0.17, but no significant difference between them.Research Conclusions Obesity resulted from high-fat diet can raise the levels ofvisfatin in the tissues of liver and heart significantly; Aerobic exercise promotes the expression of visfatin in liver and heart significantly.But obesity and aerobic exercise seem to have little effect on the expression of visfatin in quadriceps femoris muscle, just a tendency of decline, which reminds that visfatin mainly expresses in visceral tissue, while little in quadriceps femoris that needs further research.