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hTERTC27,a 27 kDa C-terminal polypeptide,can induce telomere dysfunction and inhibit tumor cell proliferation.To study the synergistic anti-tumor effects of hTERTC27 polypeptide driven by Egr-1 promoter and chemotherapeutics 5-flurorouracil (5-FU) on nasopharyngeal carcinoma (NPC),hTERTC27 eDNA was constructed to the downstream of the Egr-1 promoter,and a series of in vitro and in vivo experiments were performed.The results showed that hTERTC27 expression was significantly increased up to 7.21 folds by 5-FU activated Egr-1 promoter in C666-1 cells.Over expressed hTERTC27 made the cells more sensitive to 5-FU,and additionally inhibited cell proliferation about 20.41%.Combinational therapy of over expressed hTERTC27 driven by 5-FU activated Egr-1 promoter and 5-FU synergistically inhibited cell proliferation and promoted apoptosis of C666-1 cells for about 4.75 folds and 1.76 folds in comparison with sole therapy of hTERTC27 or 5-FU in vitro.In vivo experiments showed that over expressed hTERTC27 driven by 5-FU activated Egr-1 promoter and 5-FU synergistically reduced tumor volume,tumor weight and local infiltration,which may be relative to tumor cell apoptosis.These results suggest that combinational therapy of over expressed hTERTC27,which is driven by 5-FU activated Egr-1 promoter,and 5-FU may provide a novel approach to treat nasopharyngeal cancer.