【摘 要】
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Lentinula edodes,one of the most popular edible mushroom species with high nutrition and special flavor,is widely cultivated in many Asian countries,especially in China,Japan and Korea.As a white rot
【机 构】
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Institute of Applied Mycology,Plant Science and Technology College,Huazhong Agricultural University,
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Lentinula edodes,one of the most popular edible mushroom species with high nutrition and special flavor,is widely cultivated in many Asian countries,especially in China,Japan and Korea.As a white rot fungus with strong wood degradation ability,L edodes has the potential application in bioenergy production.However,genes,pathways and mechanisms regarding this function are still unknown.Here,we report its 41.8-Mb genome,encoding 14,889 predicted genes,obtained via next-generation sequencing technology.L edodes phylogenies and wood-degrading machinery were investigated by a comparative analysis with the model species of fungi.A total of 93 genes predicted to encode transcription factors and 41 genes related to lignocellulose degradation are up-regulated when L edodes mycelia were cultured in sawdust medium versus CYM medium.These differentially expressed genes may play important roles in lignocellulose degradation,and the research of these genes may be beneficial to bioenergy industry environmental improvement and mushroom production.The overall results will facilitate a better understanding of the molecular mechanism of lignocellulose degradation and provide the basis for partial replacement of wood sawdust with agricultural wastes in L.edodes cultivation.
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