【摘 要】
：
目的：探讨重组人睫状神经营养因子rhCNTF对体外培养的海马神经元受Aβ25-35损伤的保护作用.方法：采用原代分离、纯化wistar乳鼠海马组织,进行体外培养海马细胞.并采用人神经特异烯醇化酶(NSE)免疫细胞化学鉴定海马细胞.分别加入不同浓度的rhCNTF和10μmol/L的聚集态Aβ25-35作用于体外培养的海马细胞.采用CCK-8法观察并以成组设计分析rhCNTF对海马细胞受Aβ25-35
【机 构】
：
Department of Clinical Pharmacology, Affiliated Hospital of Academy of Military Medical Sciences, Be
论文部分内容阅读
目的：探讨重组人睫状神经营养因子rhCNTF对体外培养的海马神经元受Aβ25-35损伤的保护作用.方法：采用原代分离、纯化wistar乳鼠海马组织,进行体外培养海马细胞.并采用人神经特异烯醇化酶(NSE)免疫细胞化学鉴定海马细胞.分别加入不同浓度的rhCNTF和10μmol/L的聚集态Aβ25-35作用于体外培养的海马细胞.采用CCK-8法观察并以成组设计分析rhCNTF对海马细胞受Aβ25-35损伤的存活率影响.结果：免疫细胞化学成功鉴定了海马神经元.倒置显微镜下观察到rhCNTF处理组较rhCNTF+Aβ25-35组细胞的生长状态更好.CCK-8法结果显示与对照组比较,随着rhCNTF浓度增高,海马细胞的存活率增高,且呈幂函数相关,决定性系数R2=0.997；与rhCNTF+Aβ25-35组的存活率(如0.369±0.012)比较,rhCNTF组的海马细胞存活率(如0.394±0.015)均显著增高(P＜0.01).结论：rhCNTF对体外培养的海马细胞有神经营养作用,对Aβ25-35损伤的造成的AD模型具有神经保护作用.
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