【摘 要】
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OBJECTIVES To investigate the molecular epidemiology of genes that confer resistance to fluoroquinolones and extended spectrum β-lactams in non-typhoidal Salmonella(NTS)from food producing animals in
【机 构】
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College of Veterinary Medicine,National Reference Laboratory of Veterinary Drug Residues,South China
【出 处】
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中国药理学会第十一届全国化疗药理学术研讨会
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OBJECTIVES To investigate the molecular epidemiology of genes that confer resistance to fluoroquinolones and extended spectrum β-lactams in non-typhoidal Salmonella(NTS)from food producing animals in China.METHODS Thirty-one non-duplicate NTS were examined by PCR for plasmid mediated ESBL(TEM,SHV,OXA,CMY,and CTX-M-9),fluoroquinolone-resistance(qnr,aac(6)-Ib-cr,qepA and oqx)genes.The QRDR of topoisomerase genes (gyrA,gyrB,parC and parE)was also sequenced.Genetic relatedness of isolates was determined by PFGE;susceptibility to antibiotics was measured with CLSI methods.RESULTS Numerous combinations of different antibiotic resistance genes and mutations in topoisomerase genes were detected.Of most concern was that different isolates of Salmonella each contained several antibiotic resistance genes which together conferred high level resistance to extended spectrum β3-lactams and fluoroquinolones.CONCLUSIONS These data suggest that contamination of food by multi-drug resistant bacteria in exporting countries with highly mobile populations will lead to global transfer of new antibiotic resistances from country to country by humans or on food.Human infections by such strains would be hard to treat.
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