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Wheat powdery mildew is one of the main wheat diseases in China, seriously affecting the wheat production and quality in many provinces.In order to provide theoretical guide for wheat breeding and targeted control of the disease, the race population and virulence dynamics of Blumeria graminis f.sp.tritici were analyzed using 73 samples collected from different regions of northeastern China in 2013.114 isolates were isolated and 23 physiological races were identified, with high-virulence race group having the highest frequency.The dominant race was No.311, with a frequency 29.8%, and the sub-dominant race was No.331, with a frequency 12.3%.The results of virulence analysis showed that the occurrence frequencies of V1, V3c, V3e, V3f, V5, V6 (Coker747), /7, V8, V1 +2+9, /3a,V3d, V4a, V4b, V6 (Tingalen), V23, V30, V4 + 8, V4b + mli were higher (more than 50%),and the utilization value of their corresponding resistance genes was relatively poor;the occurrence frequencies of V2, V2 +mld, V13, V16, V21, were lower (less than 20%), their corresponding resistance genes Pm2, Pm2 + mld, Pm13, Pm16, Pm21, Pm22 have good resistances to the popular races and can be used as effective resistance genes, especially Pm21 and Pm22 were immune to all the strains tested.Additionally, the cluster analysis for virulence diversity of B.graminis f.sp.tritici from different areas was carried out using NTSYS2.10 statistical software.These results showed that population variation existed in different years, and there was also a big difference in the virulence of B.graminis f.sp.tritici from different regions.