苦皮藤(Celastrus angulatus Max.)是卫矛科南蛇藤属的一种多年生灌木,广泛分布在我国长江和黄河流域的丘陵浅山区。作为传统的杀虫植物,苦皮藤根皮具有杀虫活性,可以防治某些蔬菜害虫。为充分利用我国的植物资源,进一步开发苦皮藤的药用部位,寻找苦皮藤新的活性成分,我们系统研究了苦皮藤根皮的化学成分。应用多种现代分离纯化方法和技术,从苦皮藤根皮的乙醇提取物中分离得到17个化合物,通过各种波谱技术,包括:1H-NMR、13C-NMR、EI-MS、1H-1H COSY、HSQC、HMBC、NOESY等鉴定了其中15个化合物,成分鉴定为:β-谷甾醇(β-sitosterol,G-01);1β,2β-二乙酰氧基-8α,13-二异丁酰氧基-9β-苯甲酰基-4α,6α-二羟基-β-二氢沉香呋喃(1β,2β-diacetoxy-8α,13-diisobutanoyloxy-9β-benzoyloxy-4α,6α-dihydroxy -β–dihydroagarofuran,G-02);1β,2β,6α-三乙酰氧基-4α-羟基-8β,13-二异丁酰氧基-9α-苯甲酰氧基-β-二氢沉香呋喃(1β,2β,6α-triacetoxy-8β,13-diisobutanoyloxy-9α-benzoyloxy-4α-hydroxy-β–dihydroagarofuran,G-03);1β,2β,6α-三乙酰氧基-4α-羟基-8β-异丁酰氧基-9α-苯甲酰氧基-13-异戊酰氧基-β-二氢沉香呋喃(1β,2β,6α-triacetoxy -8β-isobutanoyloxy-9α-benzoyloxy-13-(α-methyl)-butanoyloxy- 4α-hydroxy-β-dihydroagarof -uran,G-04);1β-异烟酰氧基-2β,6α-二乙酰氧基-4α-羟基-8β,13-二异丁酰氧基-9α-呋喃酰氧基-β-二氢沉香呋喃(1β-picolinoyloxy-2β,6α-diacetoxy-8β,13-diisobutanoyloxy- 9α-furanoyloxy-4α-hydroxy-β–dihydroagarofuran,G-05);1β-异烟酰氧基-2β,6α-二乙酰氧基-4α-羟基-8β, 13-二异丁酰氧基-9α-苯甲酰氧基-β-二氢沉香呋喃(1β-picolinoyloxy-2β,6α-diacetoxy-8β,13-diisobutanoyloxy-9α-benzoyloxy-4α-hydroxy-β–dihydroagarofuran,G-06);1β,6α-二乙酰氧基-2β-烟酰氧基-4α-羟基-8β,13-二异丁酰氧基-9α-苯甲酰氧基-β-二氢沉香呋喃(1β,6α-diacetoxy-2β-nicotinoyloxy-8β,13 -diisobutanoyloxy-9α-benzoyloxy-4α-hydroxy-β–dihydroagarofuran,G-07) ;1β,2β,6α-三乙酰氧基-4α-羟基-8β,13-二异戊酰氧基-9α-苯甲酰氧基-β-二氢沉香呋喃(1β,2β,6α-triacetoxy-8β,13-di(α-methyl)-butanoyloxy-9α-benzoyloxy-4α-hydroxy-β–dihydroagarofuran,G-08);槲皮素(quercetin,G-09);儿茶素(catechin,G-10);表儿茶素(epicatechin,G-11);β-胡萝卜苷(β-daucosterol,G-12);异槲皮苷(isoquercitrin,G-13);间苯二酚(m-dihydroxybenzene,G-14);卫矛醇(dulcitol,G-15)。其中化合物G-05、G-06、G-07为新化合物,G-08为首次从该植物中分离得到。