研究黄芪苷Ⅳ(astragaloside IV, AST)抗H2O2诱导的H9c2细胞氧化损伤的作用,探讨该作用是否通过ERK1/2信号转导通路实现。用含10%FBS的高糖DMEM培养基培养H9c2细胞24 h,换用无FBS的高糖DMEM培养基,用100,200,400μmol/L的H2O2处理细胞3,6,12h,采用MTT法检测细胞存活率。选择适宜的H2O2浓度和作用时间,建立H2O2诱导H9c
The traditional fuzzy logic system (FLS) can only model and control the process in two-dimensional nature.Many of real-world systems are of multidimensional fea