Type 2 diabetes mellitus（T2DM）is a complex,progressive disease affecting an estimated420 million people worldwide.It is responsible for over 90%of people with diabetes.GLP-1（glucagon-like peptide-1）has a biological function that can help diabetes treatment by increasing insulin production.Bovine Serum Albumin is the main common plasma protein,with a half-life of nineteen days,and when utilized as an agonist,it helps prevent diabetic complications.Fullerenols,on the other hand,have been reported to have antidiabetic efficacy.This work tested fullerenol in vitro on GLP-1,BSA,and MIN6 cells.C60（OH）n effects on GLP-1 and BSA were studied utilizing spectroscopy（fluorescent and UV-Vis’s absorption spectroscopy）and molecular docking techniques.Meanwhile,the antioxidant and ROS scavenging effects of C60（OH）n on hydrogen peroxide-induced oxidative stress in MIN6 cells was investigated using MTT and ROS Assay to understand better C60（OH）n antioxidant and ROS scavenging ability in the H2O2-induced cell.In the ultraviolet-visible spectrum,the combination of GLP-1 and fullerenol has the most significant absorption peak at 276 nm.There was no discernible difference in peak intensity or position relative to GLP-1 maximum absorption peak.The fluorescence experiment used excitation and emission wavelengths of 280 nm and 348 nm,respectively.The C60（OH）n-GLP-1 complex was impacted by changes in fullerenol concentrations,with lower fluorescence spectrum intensities as C60（OH）n concentration increased.The binding constant（Ka）and the binding site were both affected by temperature,according to our findings（n）.With increasing temperature,the binding constant（Ka）increased from 4.168±0.252(L.mol-1)at 273K to 4.183±0.135(L.mol-1)at 310 K;however,the binding site reduced from 0.864±0.05 at 273K to 0.854±0.03 at 310K.The recorded free energy（G0）was negative,indicating spontaneous exchanges,further confirmed by molecular docking.Dynamic quenching was the effect investigated.GLP-1’s inherent fluorescence was suppressed by fullerenol,according to our findings.For BSA,the findings indicate that the endogenous fluorescence suppression of serum albumin generated by C60（OH）nby reversible binding to produce the ground-state complex is mediated by reversible binding to the ground-state complex.It was established that static quenching and dynamic quenching were involved in the quenching process at low concentrations.Static and dynamic quenching coexisted according to the fluorescence study;the Ksv values increased with increased temperature indicating dynamic quenching.In contrast,the collisional quenching constant（Kq）was higher than 2×1010 L·mol-1·s-1,indicating static quenching,making the BSA quenching process dynamic and static.The vd W force and hydrogen bonding were the major forces influencing BSA-C60（OH）n binding,and the reaction occurred spontaneously,which was confirmed by the molecular docking study.MTT and ROS detection in MIN6 cells provided that H2O2-induced MIN6 cell death was improved by the C60（OH）n after 48hrs of incubation,fullerenol promoted cell viability in the mouse insulinoma cell as compared to the control at a low concentration of 300μM.The ROS（Reactive Oxygen Species）detection in the cell shows that C60（OH）n significantly reduced the H2O2 elevated ROS levels in the MIN6 cell after 24 h of incubation at a low concentration of 30μM.The above results confirmed that GLP-1 and BSA inherent fluorescence were suppressed by fullerenol.Therefore,fullerenol as a novel therapy can be used for type 2 diabetes mellitus when administered as a GLP-1R agonist.It also improved cell viability and reduced ROS in MIN6cells,making it a good antioxidant and ROS scavenger which can be used as an antidiabetic therapy in treating diabetes complications.