Soybean (Glycine max L.Merr.)is oneof the most prominent legume crops playingsignificant role in worlds food security.Water stress is one of the mostsevere constraintson crop production.Present study investigated the effects of drought stress imposed forvarious periods on antioxidant activities and hormones biosynthesis in (drought treated)　　and control (non-drought treated), fourSoybeans (Glycine max L.Merr.) cultivars viz.(jindou 74,jindou 78) as droughttolerant and (H228, B217) as drought sensitive.Considerable variations in the behavior of antioxidants were observed under droughtstress in all Soybeans cultivars.Exposure of plant to drought stress considerably increaseMDA concentrations in leaves of all cultivars, and such an increment was more indrought sensitive cultivars.The ROS were scavenged by the enhanced activities ofenzymatic (SOD, CAT,POD and APX) antioxidants inresponse to drought stress withthe passage of time; therefore, maximum values for these attributes were observed at 8　　days of drought for all cultivars.Theseantioxidant activities were higher in droughttolerant cultivars as compared todrought-sensitive cultivars at various time points.Moreover, Activities of hormones (ABA,IAA,JA and SA) biosynthesis were enhancedwith the passage of time.Data depicted that drought stress substantially affected thehormone levels inall Soybean cultivars.Variations were apparent among cultivarsregarding theirresponse to drought stress.The (jindou 74,jindou 78) drought tolerantcultivarsremained superior to sensitive cultivars regarding hormone levels andantioxidantactivities.In summary, our results are suggested that manipulation of these　　antioxidants and hormones may lead to improvement in drought stress tolerance.　　Generally, one of the largest groups of plant-specific transcription factorsis NACsproteins,[(NAM) no apical meristem,[ATAF1/2] Arabidopsis transcription activationfactor and (CUC2) cup-shaped cotyledon] which play a vital role inplant growth,development and adaption to the environmental conditions.Due to multidimensional roleof NAC proteins,itis very import to identify and characterize this proteins family insoybean.In present study, 139 Gin.NACs genes were identified and analyzed,phylogenetictreewas constructed, genes structures,genome localizations, domainduplications andgenes expression summaries in soybean.The phylogenic analysisrevealed that theNACs genes could be categorized broadlyinto 18 groups.Thechromosomal localizations and genome duplication indicated139 Gm.NACs contained747 homologues and distributed across 20 chromosomes.There were similar genesstructures in conformity with Gm.NACs groups between 190 and678 in proteinsequences lengths (average length 362.7).The motifs composition of Gin.NACs washighly conserved within the same group.Most of the significantly affected genes bydrought were concentrated on chromosome 6 based on transcdptomic changes evaluationby digital geneexpression (DGE)in hairy roots.Furthermore,Gm.NAC005,Gm.NAC020,Gm.NAC070 and Gm.NAC117 demonstrated significantlyhigherexpression changes under drought treatments via quantitative real-timePCR(RT-qPCR)analyses.Taken together, 139 NACs genes in Soybean wereidentifiedwith a carefulinvestigation of their location,structure, duplication, and evolution.Likewise, somegroups have evolved,resulting in high levels of functional divergence.Interestingly,Gm.NACs genes respond differently to drought stress which indicates the importance of thisgene family in abiotic stress conditionsand may be helpful in understanding the complexmechanisms of drought stress.