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目的 CyclinD和CyclinE的异常表达常引起细胞周期调节异常而导致肿瘤。为阐明这些Cyclin基因在人体胃癌发生、发展过程中的作用 ,本文对 38例胃癌患者、2 9例胃癌的第一代亲属及 18例健康对照者进行了研究。方法 本研究对上述不同组别及相应的病理组织学表现 ,包括肠化生、萎缩、HP感染及胃炎的状况进行了Cyclin基因表达的研究 ,采取逆转录聚合酶链反应 (RT PCR)的方法。结果 胃癌组织中CyclinD2、D3和E的表达相对于癌旁正常组织明显增高 ,并且CyclinD2和D3的增高表达也发现于胃癌的第一代亲属中 (P <0 0 5 )。不同病理组织学改变 ,肠化生组织CyclinD2、D3和E的表达明显增高 ,胃粘膜萎缩仅CyclinD3表达增高 ,HP感染较未感染组CyclinD2、D3表达增高。但CyclinD1的表达状况与病理组织学改变无关。结论 本研究提示增高的CyclinD2、D3和E是胃癌变过程中的一个常见和早期事件 ,并且基因的异常表达可出现于胃癌患者的第一代亲属中。CyclinD1的表达与胃癌变过程中的临床及病理学变化无明显关系。
Purpose Abnormal expression of CyclinD and CyclinE frequently cause abnormal cell cycle regulation and lead to tumor. To clarify the role of these Cyclin genes in the development and progression of human gastric cancer, 38 gastric cancer patients, 29 first-degree relatives of gastric cancer and 18 healthy controls were studied. Methods In this study, Cyclin gene expression in different groups and corresponding histopathological manifestations, including intestinal metaplasia, atrophy, HP infection and gastritis were studied. RT-PCR was used . Results The expression of CyclinD2, D3 and E in gastric cancer tissues was significantly higher than that in adjacent normal tissues, and the increased expression of CyclinD2 and D3 was also found in the first generation of gastric cancer (P <0.05). The expression of CyclinD2, D3 and E in intestinal metaplasia was significantly increased, the expression of CyclinD3 increased only in gastric mucosal atrophy, and the expression of CyclinD2 and D3 in HP infection was higher than that in non-infected group. However, the expression of CyclinD1 has nothing to do with the histopathological changes. Conclusions This study suggests that elevated CyclinD2, D3 and E are common and early events in gastric carcinogenesis, and aberrant gene expression may occur in the first-generation relatives of gastric cancer patients. The expression of CyclinD1 had no significant relationship with the clinical and pathological changes in gastric carcinogenesis.