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目的:研究促红细胞生成素(EPO)在中枢神经系统血管母细胞瘤(HB)中的表达及其意义。方法:收集14例HB患者(7例囊性、7例实质性)术前血清及其中5例囊性HB的囊液、3例实质性HB的原代培养上清液以及11名正常志愿者的血清,用化学发光免疫法测定样品EPO浓度,同时对3例实质性HB体外培养细胞行EPO免疫荧光染色。结果:囊性HB的囊液中EPO浓度明显升高;实质性HB原代培养上清液中也检测到EPO的表达;实质性HB体外培养细胞中基质细胞胞质对EPO呈阳性表达;HB患者术前血清中EPO浓度与正常对照组相比差异无统计学意义。结论:HB中的基质细胞可以合成和分泌EPO,血清EPO检测对诊断HB没有参考价值。
Objective: To study the expression of erythropoietin (EPO) in central nervous system hemangioblastoma (HB) and its significance. Methods: Preoperative sera from 14 patients with HB (7 cystic and 7 essential) preoperative serum and 5 cystic HB cyst were collected, 3 primary HB supernatant and 11 normal volunteers EPO concentration was determined by chemiluminescence immunoassay. At the same time, EPO immunofluorescence staining was performed on 3 HBs cultured in vitro. Results: The concentration of EPO in cystic fluid of cystic fluid was significantly increased. The expression of EPO was also detected in the primary culture supernatant of HB. The expression of EPO was positive in the cytoplasm of stroma of HB in vitro. HB The preoperative serum EPO concentration in patients with no significant difference compared with the normal control group. Conclusion: The stromal cells in HB can synthesize and secrete EPO. The detection of serum EPO has no reference value for the diagnosis of HB.