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目的:观察由编码HBsAg与HCV-CE_2原的两种重组真核细胞表达质粒制备的DNA疫苗联合接种BALB/c小鼠后,其诱生特异性免疫应答的规律和相互影响。方法:应用上述2种DNA疫苗单次联合免疫小鼠,动态观察血中特异性抗体水平;并完成稳定转染,表达相应抗原的SP2/0骨髓瘤细胞的建株,采用CTL杀伤活性体内诱生实验的方法,建立观察DNA疫苗免疫保护与治疗效应的动物模型。结果:两种DNA疫苗单次联合免疫小鼠,能够共同诱生特异性机体免疫应答,但特异性抗体阳性率及滴度均不高;而CTL杀伤活性体内实验的结果则显示,经DNA疫苗联合接种免疫的小鼠荷瘤目的靶细胞后,其生存率明显高于未免疫鼠(P<0.05)。接种后特异性抗体呈阴性的小鼠也表现出相似的CTL保护效果。结论:DNA疫苗诱生的CTL应答可与体液免疫应答分离存在,而且可能是DNA疫苗免疫保护效应更重要的方面。
OBJECTIVE: To observe the regularity and interaction of specific immune responses induced by DNA vaccines prepared from two recombinant eukaryotic expression plasmids encoding HBsAg and HCV-CE_2 in BALB / c mice. Methods: The mice were immunized with the above two kinds of DNA vaccines to observe the levels of specific antibodies in blood. The SP2 / 0 myeloma cells stably transfected and expressing the corresponding antigens were established and induced by CTL. Health experimental methods, the establishment of observation DNA vaccine immunoprotective and therapeutic effects of animal models. Results: The single immunization with two kinds of DNA vaccine could induce the specific immune response together, but the positive rate and titer of the specific antibody were not high. However, the results of in vivo experiments of CTL killing activity showed that DNA vaccine The survival rate of the co-immunized mice bearing tumor-bearing target cells was significantly higher than that of the non-immunized mice (P <0.05). Mice that were negative for specific antibodies after vaccination also showed similar CTL protection. CONCLUSION: CTL responses induced by DNA vaccines may be separated from the humoral immune response and may be a more important aspect of the immunoprotective effect of DNA vaccines.