论文部分内容阅读
本研究观察急性白血病患儿外周血CD4+ CCD25+调节性T细胞(CD4+ CD25+ Treg)及自然杀伤细胞(nature killer cell,NK)在不同病程阶段的变化,了解白血病患儿的免疫状态,以探讨CD4+ CD25+ Treg细胞及NK细胞在急性白血病肿瘤免疫中的意义。建立流式细胞术检测外周血CD4+ CD25+ Treg细胞和NK细胞的方法;检测急性白血病初诊患儿25例、完全缓解患儿28例及20例正常健康对照者外周血CD4+ CD25+ Treg细胞及NK细胞的数量及比例。结果表明:初诊组、完全缓解组及对照组外周血CD4+ CD25+ CD127+占CD4+ T细胞的比例分别为(9.55±2.41)%,(8.54±2.51)%和(6.25±0.85)%,在初诊患儿组和缓解患儿组高于正常对照组,且在初诊患儿组高于完全缓解组(p(0.05);同时,与正常对照组比较,急性白血病患儿的NK细胞数量减少,完全缓解后患儿组NK细胞数量仍低于正常对照(4.11±3.87%和10.41±7.20%vs14.06±5.95%,p(0.05)。结论:联合应用CD4、CD25及CD127检测Treg细胞简便可行、重复性好、检测结果可靠、准确,CD4+ CD25+ CD127+ T细胞可较好地反映CD4+ CD25+ Treg细胞的比例。急性白血病患儿外周血中Treg细胞数量升高,NK细胞数量降低,表明急性白血病患儿NK细胞免疫功能处于抑制状态。Treg细胞可能在白血病的发生、发展中起一定作用,参与NK细胞的调节可能是Treg细胞在白血病免疫中的一个环节。
In this study, the changes of CD4 + CD25 + Treg and NK cells in peripheral blood of children with acute leukemia were observed in different stages of the course of the disease, to understand the immune status of children with leukemia, to explore the CD4 + CD25 + Treg and natural killer cell (NK) Significance of Treg cells and NK cells in the tumorigenesis of acute leukemia. To establish a method for detecting CD4 + CD25 + Treg cells and NK cells in peripheral blood by flow cytometry. Twenty-five patients with newly diagnosed acute leukemia, complete remission of CD4 + CD25 + Treg cells and NK cells in peripheral blood from 28 healthy children and 20 healthy controls Number and proportion. The results showed that the percentage of CD4 + CD25 + CD127 + CD4 + T cells in peripheral blood of newly diagnosed group, complete remission group and control group were (9.55 ± 2.41)%, (8.54 ± 2.51)% and (6.25 ± 0.85)%, Group and remission group were higher than those in normal control group, and were higher in newly diagnosed group than in complete remission group (p <0.05). At the same time, compared with normal control group, the number of NK cells in children with acute leukemia was reduced, and after complete remission The number of NK cells in children group was still lower than that in the control group (4.11 ± 3.87% vs 10.41 ± 7.20% vs 14.06 ± 5.95%, p (0.05) .Conclusion: The combination of CD4, CD25 and CD127 detection Treg cells is simple and reproducible CD4 + CD25 + CD127 + T cells can reflect the proportion of CD4 + CD25 + Treg cells in children with acute leukemia.The number of Treg cells and the number of NK cells in peripheral blood of children with acute leukemia are decreased, indicating that NK cells in children with acute leukemia Treg cells may play a role in the occurrence and development of leukemia, and participation in the regulation of NK cells may be a part of Treg cells in leukemia immunity.