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探讨白念珠菌氟康唑作用的靶酶编码基因 (ERG11)突变与耐药性的关系。分离抽提分别对氟康唑敏感和耐药的白念珠菌基因组 DNA,根据 ERG11编码序列设计一对上下游引物 ,对靶酶活性中心血色素结合区和疏水端螺旋区的基因表达序列进行 PCR扩增 ,经 DNA测序比较敏感和耐药菌株的碱基差异。结果 :6株氟康唑耐药白念珠菌此段序列有 5个位点发生突变 ,其中 5株第 1344 bp位点 G→A的突变致所编码的第 44 8个氨基酸由甘氨酸 (G)变为谷氨酸 (E)。结论 :ERG11基因突变致所编码的氨基酸改变与氟康唑耐药有关。
To investigate the relationship between Candida albicans fluconazole target gene mutation (ERG11) and drug resistance. Isolation and extraction of fluconazole-resistant Candida albicans genomic DNA, according to the ERG11 coding sequence design of a pair of upstream and downstream primers, target enzyme activity center hemopexin binding region and the hydrophobic region of the helicase gene expression sequence PCR amplification Increased by the DNA sequencing more sensitive and resistant strains of the base difference. Results: Five strains of Candida albicans resistant to fluconazole were mutated in five loci. Among them, the mutation of G → A at position 1344 of five strains resulted in the mutation of glycine (G) Change to glutamic acid (E). CONCLUSION: The amino acid changes caused by mutations in ERG11 gene are associated with fluconazole resistance.