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中国药典1995年版一部赤芍中芍药甙的含量测定方法为TLC-紫外分光光度法。我们在实验中发现该方法重现性差,为此我们对其含量测定中的提取方法做了比较实验。 1 仪器与试药日本岛津UV-240紫外分光光度计,芍药甙对照品(中国药品生物制品检定所提供),硅胶GF_(254)(青岛海洋化工厂),所用试剂勾分析纯。 2 方法与结果 2.1 药典法按1995年版中国药典一部(142页)方法测定。 2.2 回流提取法精密称定样品中粉约2.5g,置100ml回流瓶中,加入甲醇50m1,称定。置水浴上回流提取2h,放冷,称定,用甲醇补足损失的重量,摇匀,滤过,取续滤液按中国药典法测定。共测定赤芍样品5批,结果见附表。
The Chinese Pharmacopoeia of 1995, a method for the determination of paeoniflorin in Radix Paeoniae Rubra is TLC-UV spectrophotometry. We found that the method was poorly reproducible in the experiment. For this reason, we conducted a comparative experiment on the extraction method for its content determination. 1 Instrument and reagent Japan Shimadzu UV-240 UV spectrophotometer, paeoniflorin reference product (provided by the China Institute of Pharmaceutical and Biological Products), silica gel GF_ (254) (Qingdao Marine Chemical Factory), the reagent reagent used in the analysis of pure. 2 Methods and Results 2.1 The Pharmacopoeia Law was determined according to the 1995 Chinese Pharmacopoeia (142 pages) method. 2.2 reflux extraction method accurately weighed about 2.5g sample powder, set in a 100ml reflux bottle, add methanol 50m1, weighed. The water bath was refluxed and extracted for 2 hours, let cool, weighed, and the weight of the loss was made up with methanol, shaken, filtered, and the filtrate was measured according to the Chinese Pharmacopoeia Law. A total of 5 samples of Radix Paeoniae Rubra were assayed. The results are shown in the attached table.