To improve the measurement accuracy of structured laser for inner surface dimensions of a deep hole, a new method to extract the laser stripe center line is proposed. An improved adaptive genetic algorithm that can converge rapidly and search the global optimum is used to determine the threshold for the laser stripe segmentation. And then NURBS interpolation which has a good local control capability is adopted to extract the laser stripe center line. Experiments show that the extracted laser stripe center line is stable and the diameter of the deep hole can be measured accurately.