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为探讨白细胞介素 13(IL 13)在急性肾小球肾炎 (AGN )发病机制中的作用 ,应用逆转录 多聚酶链反应 (RT PCR )及ELISA法检测了 2 4例AGN患儿外周血单个核细胞 (PBMC )中IL 13mRNA和蛋白水平的变化。结果表明 ,AGN急性期IL 13mRNA和蛋白水平显著高于正常对照组 ,恢复期降至正常水平。重症患儿急性期IL 13mRNA和蛋白水平显著高于轻症患儿。IL 13水平与血清补体C3和尿红细胞计数呈显著相关关系。本研究结果提示IL 13在AGN中可能具有一定的抗炎作用。
To investigate the role of interleukin-13 (IL-13) in the pathogenesis of acute glomerulonephritis (AGN), the peripheral blood mononuclear nuclei of 24 AGN children were detected by reverse transcriptase-polymerase chain reaction (RT PCR) and ELISA IL-13 mRNA and protein levels in cells (PBMCs). The results showed that the level of IL-13 mRNA and protein in acute AGN stage was significantly higher than that in normal control group, and the recovery period was reduced to normal level. IL 13 mRNA and protein levels in critically ill children during acute phase were significantly higher than those in mild cases. There was a significant correlation between IL-13 level and serum complement C3 and urinary erythrocyte count. Our results suggest that IL 13 may have some anti-inflammatory effects in AGN.