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建立一种一步法和二温式的逆转录聚合酶链式反应,用于扩增南美白对虾桃拉综合症病毒231bp的特异基因片断,对300份南美白对虾临床样品的检测结果表明,这是一种快速和敏感的桃拉综合症病毒诊断方法。
To establish a one-step and two-temperature reverse transcriptase-polymerase chain reaction (PCR) for the amplification of 231bp specific gene fragment of Taraxaci panxeniae, the detection results of 300 clinical samples of P. vannamei showed that Is a fast and sensitive method for the diagnosis of Taura syndrome virus.