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力生长因子(mechano-growth factor,MGF)是新近发现的一种诱导性表达的生长因子,由IGF-1基因选择性剪接产生,力生长因子E肽(MGF-24E)是MGF区别于其它IGF-1剪接体的特异性C端序列,其在预防组织修复等方面有重要作用.采用Ad-Easy法成功构建MGF,MGF-24E重组腺病毒,重组腺病毒分别感染神经胶质细胞N9和神经元细胞SH-SY5Y,MTT法检测其对两种细胞增殖的影响.结果表明,经腺病毒介导可实现MGF和MGF-24E在神经细胞中的高表达,与对照组相比,MGF和MGF-24E在时间和浓度上对促进N9和SH-SY5Y细胞的增殖均有显著性效应,且二者效果相当.由此认为,MGF和MGF-24E具有促进神经损伤修复的同等作用力,为力生长因子用于基因治疗提供更为切实的理论基础.
Mechanistic-growth factor (MGF) is a recently discovered inducible growth factor that is produced by alternative splicing of the IGF-1 gene. MGF-24E is a marker that distinguishes MGF from other IGFs -1 splicing has a significant role in the prevention of tissue repair, etc. The recombinant adenovirus MGF and MGF-24E were successfully constructed by Ad-Easy method, and the recombinant adenovirus was infected with N9 and N9 The effects of MGF and MGF-24E on the proliferation of the two cell lines were detected by SH-SY5Y and MTT assay.The results showed that MGF and MGF-24E were highly expressed in neurons after adenovirus mediated treatment.Compared with the control group, MGF and MGF -24E had a significant effect on promoting the proliferation of N9 and SH-SY5Y cells both in time and concentration, and both of them had the same effect.It is considered that MGF and MGF-24E have the same force of promoting nerve injury repair, Growth factors provide a more practical rationale for gene therapy.