目的合成能够与碳酸酐酶9(carbonic anhydraseⅨ,CAⅨ)蛋白多糖区特异性结合的多肽分子(proteoglycan-like region peptide,PGLR-P1),并初步探讨其在体外的寻靶能力。方法通过化学固相合成法合成多肽分子,对其纯度和相对分子质量进行分析,并初步探讨其与CAⅨ蛋白、表达CAⅨ的肿瘤细胞和相应移植瘤组织的结合能力以及携载多肽的靶向纳米泡的体外寻靶能力。结果合成的多肽分子纯度高于98%,相对分子质量(1 722.2±0.4),其能与CAⅨ蛋白、表达CAⅨ的肿瘤细胞及相应移植瘤组织发生特异性靶向结合,且携载多肽的靶向纳米泡特异性靶向结合CAⅨ表达阳性的肿瘤细胞。结论化学合成的多肽分子PGLR-P1在分子、细胞和组织水平均能与CAⅨ蛋白发生特异性结合。 OBJECTIVE: To synthesize a proteoglycan-like region peptide (PGLR-P1) capable of specifically binding to proteoglycan of carbonic anhydrase IX (CAIX) and to explore its in vitro targeting ability. Methods Peptide molecules were synthesized by chemical solid-phase synthesis and their purity and relative molecular mass were analyzed. The binding capacity of CAIX protein, CAⅨ-expressing tumor cells and corresponding xenograft tumor tissues and targeting nanoparticles Bubble in vitro targeting ability. Results The purity of the synthesized peptide was higher than 98% and the relative molecular weight was 1722.2 ± 0.4. It could specifically bind to CAⅨ protein, CAⅨ-expressing tumor cells and the corresponding xenograft tumor, Targeting nanobubbles specifically binds CANA-positive tumor cells. Conclusion The chemically synthesized peptide PGLR-P1 can specifically bind to CAⅨ protein at molecular, cellular and tissue level.