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目的观察花色苷单体矢车菊素-3-葡萄糖苷(Cy-3-G)对Aβ_(25-35)损伤SH-SY5Y细胞的保护作用,并初步探讨相关机制。方法体外培养SH-SY5Y细胞。实验分组:首先进行Aβ_(25-35)损伤浓度的筛选,将细胞分为对照组和不同浓度Aβ_(25-35)处理组(浓度为5、10、15、20、25μmol/L);在确定Aβ_(25-35)的损伤浓度基础上进行Cy-3-G干预浓度的筛选,将细胞分为对照组、Aβ_(25-35)处理组和不同浓度Cy-3-G预孵育组(浓度为10、25、50、100μmol/L);而后进行Cy-3-G干预时间的筛选,将细胞分为对照组、Aβ_(25-35)处理组和不同时间Cy-3-G预孵育组(时间为3、6、12、24h)。MTT法测定细胞活力,Hoechst染色法检测细胞凋亡率。结果与对照组比较,10μmol/L Aβ_(25-35)处理SH-SY5Y细胞时,细胞活力显著降低(P<0.05);Cy-3-G对Aβ_(25-35)处理SH-SY5Y细胞保护作用的适宜浓度和适宜作用时间分别为100μmol/L和12h;Aβ_(25-35)处理组细胞凋亡率较对照组明显升高(P<0.05),而Cy-3-G预孵育可明显抑制Aβ_(25-35)细胞凋亡。结论适宜剂量的Cy-3-G对Aβ_(25-35)损伤的SH-SY5Y细胞具有保护作用,其机制可能与抑制细胞凋亡有关。
Objective To observe the protective effect of cyanidin-3-glucoside (Cy-3-G) on SH-SY5Y cells injured by Aβ_ (25-35), and to explore its underlying mechanism. Methods SH-SY5Y cells were cultured in vitro. The experimental group: First, Aβ_ (25-35) injury concentration screening, the cells were divided into control group and different concentrations of Aβ_ (25-35) treatment group (concentration of 5,10,15,20,25μmol / L) The concentration of Aβ_ (25-35) was determined on the basis of the concentration of Cy-3-G intervention, the cells were divided into control group, Aβ_ (25-35) treatment group and different concentrations of Cy-3-G preincubation group The cells were divided into control group, Aβ_ (25-35) treated group and Cy-3-G pre-incubated at different time Group (time 3,6,12,24 h). Cell viability was determined by MTT assay and apoptosis rate was detected by Hoechst staining. Results Compared with the control group, the viability of SH-SY5Y cells was significantly decreased when treated with 10 μmol / L Aβ 25-35 (P <0.05); Cy-3-G protected SH-SY5Y cells treated with Aβ 25-35 The suitable concentration and duration of action were 100μmol / L and 12h, respectively. The apoptosis rate of Aβ_ (25-35) group was significantly higher than that of the control group (P <0.05), while Cy-3-G preincubation Inhibit Aβ_ (25-35) apoptosis. Conclusion Appropriate doses of Cy-3-G have a protective effect on SH-SY5Y cells damaged by Aβ 25-35, which may be related to the inhibition of apoptosis.