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【目的】为了了解再生水灌溉对草坪根际可培养细菌群落组成的影响,【方法】采用稀释平板法,对北京市陶然亭公园内再生水灌区及其对照自来水灌区草坪根际细菌进行了分离,并对其16S rDNA序列进行了分析。【结果】16S rDNA序列分析表明自来水样品分离得到的菌落分属于15个属的20个种,而再生水样品分离得到的菌落分属于18个属的24个种。自来水和再生水灌区草坪根际细菌主要包括变形菌门α亚群(Alphaproteobacteria,分别为9.7%和13.4%)、变形菌门β亚群(Betaproteobacteria,分别为8.1%和12.3%)、变形菌门γ亚群(Gammaproteobacteria,分别为17.9%和42.0%)、拟杆菌门(Bacteroidetes,分别为13.0%和2.9%)、厚壁菌门(Firmicutes,分别为23.6%和10.1%)和放线菌门(Actinobacteria,分别为27.6%和19.6%)其中,芽孢杆菌属(Bacillus sp.)是自来水灌区草坪根际优势菌属(23.6%),而不动杆菌属(Acinetobacter sp.)是再生水灌区根际优势菌属(17.4%)。从不同类群优势菌属看,除变形菌门γ亚群受再生水影响优势菌属在两灌区表现出一定的差异外,其余各亚群优势菌属均未受再生水影响,其中,不动杆菌属是再生水灌区变形菌门γ亚群的优势菌属(41.3%),肠杆菌属(Enterobacter sp.)是自来水灌区变形菌门γ亚群的优势菌属(45.4%)。【结论】这表明,再生水灌溉未改变细菌群落组成类型,但改变了不同类型多度分布状况。具体表现为优势种多度值增加及部分非优势种有无。再生水灌区特有机会性致病菌、植物致病菌和重金属耐性细菌的出现表明再生水灌溉中病原微生物及重金属的控制工作还有待于进一步严格。
【Method】 In order to understand the effect of reclaimed water irrigation on the composition of culturable bacterial community in turf rhizosphere, [Method] The rhizosphere bacteria in the reclaimed water irrigated area and the control tap water irrigated area in Beijing Taoranting Park were separated by dilution plate method Its 16S rDNA sequence was analyzed. 【Result】 The results of 16S rDNA sequence analysis showed that the colonies isolated from tap water samples belonged to 20 species of 15 genera, while the colonies isolated from reclaimed water samples belonged to 24 species of 18 genera. The rhizospheric bacteria in tap water and reclaimed water irrigated area mainly include Alphaproteobacteria (9.7% and 13.4% respectively), Betaproteobacteria (8.1% and 12.3% respectively), Proteus mirabilis Subgroups (17.9% and 42.0%, respectively), Bacteroidetes (13.0% and 2.9%, respectively), Firmicutes (23.6% and 10.1%, respectively) and Actinobacteria Actinobacteria were 27.6% and 19.6%, respectively. Among them, Bacillus sp. Was the rhizosphere dominant species (23.6%) in the irrigated irrigated area and Acinetobacter sp. Was the dominant rhizosphere in the irrigated irrigated area The genus (17.4%). From the dominant taxa of different taxa, all the dominant subfamilies were not affected by reclaimed water, except that the dominant subfamily γ subgroup affected by reclaimed water showed some differences in the two irrigation areas. Among them, Acinetobacter Is the dominant genus (41.3%) of the γ-subgroup of the Proteobacteria in reclaimed water irrigated areas. Enterobacter sp. Is the predominant genus (45.4%) of the γ-subgroup of the proteobacteria in tap water irrigation areas. [Conclusion] This indicates that the reclaimed water irrigation did not change the bacterial community composition type, but changed the different types of multi-level distribution. Specific performance of the dominant species increased the degree of value and some non-dominant species. The emergence of opportunistic pathogenic bacteria, plant pathogenic bacteria and heavy metal-tolerant bacteria in reclaimed water irrigated areas indicates that the control of pathogenic microorganisms and heavy metals in reclaimed water irrigation needs to be further stricter.