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噬菌体表面展示技术是一项特异性多肽或蛋白的筛选技术,它将随机序列的多肽或蛋白片段与噬菌体衣壳蛋白融合表达而呈现于病毒表面,被展示的多肽能保持相对独立的空间结构,使其能够与配体作用而达到模仿性筛选特异性分子表位,从而提供了高通量高效率的筛选系统。近年来噬菌体展示技术已广泛应用于肿瘤抗原抗体库的建立、单克隆抗体制备、多肽筛选、疫苗研制、肿瘤相关抗原筛选和抗原表位研究、药物设计、癌症检测和诊断、基因治疗及细胞信号转导研究等。就近年来噬菌体展示技术在肿瘤相关研究中的运用作以综述。
Phage display technology is a specific polypeptide or protein screening technology, it will random sequence of peptide or protein fragments and phage capsid protein fusion expression presented in the virus surface, the displayed polypeptide can maintain a relatively independent spatial structure, Allowing it to interact with ligands to achieve mimicry-specific molecular epitopes, providing a high-throughput, high-efficiency screening system. In recent years, phage display technology has been widely used in the establishment of tumor antigen antibody library, monoclonal antibody preparation, polypeptide screening, vaccine development, tumor-associated antigen screening and antigen epitope research, drug design, cancer detection and diagnosis, gene therapy and cell signaling Transduction studies. In recent years phage display technology in cancer-related research to review.