对贵州不同海拔鱼腥草中绿原酸、芦丁、槲皮素、槲皮苷、金丝桃苷、山奈素、异鼠李素、槲皮素-3-O-β-D-葡萄糖苷以及挥发性化学成分行研究,通过HPLC分析绿原酸和黄酮类化合物,挥发性化学成分利用顶空固相微萃取结合气-质联用(HS-SPME-GC/MS)进行分析,总挥发油的含量用水蒸气蒸馏提取获得。结果表明:鱼腥中绿原酸和黄酮的含量分别在海拔1400～1500m和500～600m有较高的含量;鱼腥草精油在海拔600～700m有较高的含量,甲基正壬酮含量随海拔的增加而变化在700～800m有较高的含量;单萜(蒎烯)、倍半萜(石竹烯(Ⅱ))、脂肪酮(甲基正壬酮)、脂肪酸(羊油酸)和脂肪酸酯(2,6,10,14-四甲基十五酸甲酯)的含量在不同的海拔有显著的差异。 The effects of chlorogenic acid, rutin, quercetin, quercitrin, hyperin, kaempferol, isorhamnetin, quercetin-3-O-β-D-glucoside Volatile chemical constituents were determined by HPLC. Chlorogenic acids and flavonoids were analyzed by HPLC. The volatile compounds were analyzed by headspace solid-phase microextraction combined with gas chromatography-mass spectrometry (HS-SPME-GC / MS) The content is extracted by steam distillation. The results showed that the contents of chlorogenic acid and flavonoid in fishy fish were higher at the altitude of 1400-1500m and 500-600m respectively. The content of Houttuynia cordata Thunb. In the fish was higher at the altitude of 600-700m, With the increase of altitude, the contents of monoterpenes (pinene), sesquiterpenes (caryophyllene), aliphatic ketones (methyl n-nonanone), fatty acids And fatty acid esters (methyl 2,6,10,14-tetramethylpentadecanoate) at different altitudes were significantly different.