目的 在实验室中观察紫虹消毒剂不同浓度水溶液对金黄色葡萄球菌,大肠埃希菌、枯草杆菌黑色变种芽胞、乙型肝炎病毒杀灭作用时间和紫虹水溶液在不同温度时间存放的残留浓度。方法 在20～22°C以（1 000～125）mg/L分别观察对金黄色葡萄球菌、大肠埃希菌、枯草杆菌黑色变种芽胞、乙型肝炎表面抗原的杀灭时间,并利用经典恒温加速法和初匀速法了解在不同温度下溶液存放时间的浓度稳定性。结果 紫虹消毒剂溶液125mg/L 10 min对金黄色葡萄球菌、大肠埃希菌的杀灭率100％;250 mg/L对枯草杆菌30 min杀灭率100％;1000mg/L 20 min对乙型肝炎表面抗原杀灭率 100％;紫虹消毒剂溶液在0℃保存247h、10℃保存89h、20℃保存33h、30℃保存13h,其溶液仍可达90％。结论 紫虹消毒剂是三氰异氯尿酸和苯酚（石碳酸）等复合消毒液,在实验中其杀菌效果随溶液浓度增加和时间延长而增强,溶液稳定性随温度升高和时间延长而下降。 Objective To observe the effect of different concentrations of aqueous solution of purple rainbow disinfectant on the staphylococcus aureus, Escherichia coli, Bacillus subtilis black mutant spores, the killing time of hepatitis B virus and the residual concentration of aqueous solution of purple rainbow in different temperature time . Methods The killing time of Staphylococcus aureus, Escherichia coli and Bacillus subtilis black spores and hepatitis B surface antigen were observed at (1 000 ~ 125) mg / L at 20 ~ 22 ° C, respectively. Acceleration and Uniform Velocity methods are used to understand the concentration stability of solution at different temperatures. Results The killing rate of Staphylococcus aureus and Escherichia coli was 100% at 125 mg / L for 10 h. The killing rate of Bacillus subtilis at 100 mg / L was 100% at 250 mg / L for 20 min, The killing rate of hepatitis B surface antigen was 100%; the solution of Zixin disinfectant was stored at 0 ° C for 247h, stored at 10 ° C for 89h, stored at 20 ° C for 33h, and stored at 30 ° C for 13h. The solution was still up to 90%. Conclusions Zihong disinfectant is a complex disinfectant solution of cyanuric chloride and phenol (carbolic acid). Its bactericidal effect increases with the increase of solution concentration and time, and its stability decreases with increasing temperature and time .