在不孕症治疗中,IVF技术得到了广泛的应用,同时人们也发现在体外培养中分裂的胚胎有很大部分是染色体嵌合型的,即二倍体细胞与非二倍体细胞同时存在。常规的诊断方法是染色体核型分析。本研究采用多种探针荧光原位杂交(FISH)方法,检测从2-细胞到囊胚阶段胚胎中染色体嵌合型的发生率、类型以及不同类型的嵌合型与胚胎发育及存活之间的联系。 研究对象为81例接受IVF或ICSI治疗的患者。平均年龄36 In the treatment of infertility, IVF technology has been widely used, but also found that in vitro cultured embryos split a large part of the chromosome mosaic, that diploid cells and non-diploid cells co-exist . The routine diagnostic method is karyotype analysis. In this study, a variety of probe fluorescence in situ hybridization (FISH) method was used to detect the incidence and types of chromosome chimerism in embryos from 2-cell to blastula stages and between different types of chimerism and embryonic development and survival Contact. The subjects were 81 patients treated with IVF or ICSI. Average age 36