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用低蛋白饮食方法建立豚鼠胆色素结石模型，共设对照、致石、维生素Ｃ修复、丹参修复和对照修复等５组，规定时间内处死动物，用放射免疫、固相酶联免疫、生物化学等方法检测肝细胞内环—磷酸腺甙（ｃＡＭＰ）、环—磷酸鸟嘌呤（ｃＧＭＰ）、钙调素（ＣａＭ）、钙，三磷酸腺甙酶（Ｃａ２＋－ＡＴＰａｓｅ）、磷酸化酶ａ等水平。致石组豚鼠肝脏细胞内ｃＡＭＰ和磷酸化酶ａ升高，而ｃＧＭＰ，ＣａＭ和Ｃａ２＋－ＡＴＰａｓｅ下降，表明肝细胞钙稳态呈失调状态。维生素Ｃ和丹参可调整肝细胞的上述改变，说明维生素Ｃ和丹参具有维持肝细胞钙稳态的作用。
A low-protein diet was used to establish the model of porcine bile pigment stones. Five groups including control, stone, vitamin C repair, salvia miltiorrhiza repair and control repair were set up. The animals were sacrificed within the specified time and radioimmunoassay, enzyme-linked immunosorbent assay The levels of cAMP, cGMP, CaM, Ca, Ca2 + -ATPase and phosphorylase-a in hepatocytes were detected by ELISA, . The results showed that the cAMP and phosphorylase A in guinea pigs were increased, while the cGMP, CaM and Ca2 + -ATPase in guinea pigs were decreased, which indicated that the hepatocytes calcium status was abnormal. Vitamin C and Salvia can adjust the above changes of liver cells, indicating that vitamin C and Salvia have to maintain the role of hepatocyte calcium homeostasis.