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目的: 探讨重组质粒pcDNA3. 1 IL- 15对小鼠骨髓树突状细胞(DC)表面共刺激分子的表达及免疫功能的影响。方法: 构建真核表达质粒pcDNA3. 1 IL- 15, 以其转染小鼠骨髓DC。用流式细胞仪检测转染的DC表面CD40、CD80及CD86的表达, 并分析转染的DC刺激脾淋巴细胞中CD4+、CD8+ T细胞亚群的变化。用MTT比色法检测转染的DC刺激T细胞增殖的作用。用ELISA法检测T细胞产生IFN- γ的水平。结果: pcDNA3. 1- IL- 15转染的DC表面CD40、CD80及CD86的表达均有不同程度的升高。重组质粒转染的DC可诱导小鼠脾淋巴细胞中CD4+、CD8+ T细胞增殖, 但CD4+ /CD8+ T细胞的比值降低。结论: 重组质粒pcDNA3. 1 -IL- 15转染可提高DC表面共刺激分子的表达并增强其免疫功能。
Objective: To investigate the effect of recombinant plasmid pcDNA3.1 IL-15 on costimulatory molecule expression and immune function of mouse bone marrow dendritic cells (DCs). Methods: Eukaryotic expression plasmid pcDNA3.1 IL-15 was constructed and transfected into mouse bone marrow DCs. The expression of CD40, CD80 and CD86 on the transfected DCs was detected by flow cytometry. The changes of CD4 + and CD8 + T cell subsets in splenic lymphocytes were analyzed by transfected DCs. MTT assay was used to detect the effect of transfected DC on T cell proliferation. The level of IFN-γ produced by T cells was detected by ELISA. Results: The expression of CD40, CD80 and CD86 in DCs transfected with pcDNA3.1-IL-15 all increased to some extent. Recombinant plasmids transfected DC could induce the proliferation of CD4 +, CD8 + T cells in splenic lymphocytes of mice, but the ratio of CD4 + / CD8 + T cells decreased. CONCLUSION: Transfection of recombinant plasmid pcDNA3.1-IL-15 can enhance the expression of DC costimulatory molecules and enhance their immune function.