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1976年,Lee氏应用非疫区的黑线姬鼠从疫区黑线姬鼠肺中分离出流行性出血热病毒(EHFV)。此后,应用A549(人肺癌)。Veto-E_6(绿猴肾)、2BS(人胚肺二倍体)等细胞分离EHFV相继获得成功。但应用Hep-2细胞(人喉癌细胞)从鼠肺标本中分离出EHFV尚未见报道。我们于1991年至1992年首次应用Hep-2细胞从闽南的褐家鼠肺中分离出EHFV,现报道如下。
In 1976, Lee’s non-infected Apodemus agrarius virus was isolated from the Apodemus agrarius lungs in the endemic area of the epidemic, and the epidemic hemorrhagic fever virus (EHFV) was isolated. Thereafter, A549 (human lung cancer) was applied. Veto-E_6 (green monkey kidney), 2BS (human embryo lung diploid) and other cells isolated EHFV have been successful. However, the isolation of EHFV from murine lung samples using Hep-2 cells (human laryngeal carcinoma cells) has not been reported. We first isolated Hep-2 cells from the Rattus norvegicus lungs from Minnan in 1991-1992 and are reported below.