论文部分内容阅读
目的 利用四氯化碳大鼠肝硬化模型 ,研究绿茶提取物对慢性肝损伤的保护作用。方法 将动物分为 3组 :正常对照组 ,绿茶提取物处理组 ,肝硬化组。绿茶提取物处理组及肝硬化组分别按 0 3ml/ 1 0 0g皮下注射 40 %CCl4 ,每周 2次。于注射CCl4 后的第 2周 ,第 9周分两批处死大鼠。观察肝羟脯氨酸含量、肝脂质过氧化产物丙二醛含量 (MDA)、肝组织病理变化及肝组织中转化生长因子 β1 (TGF β1 )mRNA表达情况。结果 绿茶提取物处理组与肝硬化组相比 ,第 2周及第 9周MDA含量、TGF β1 mRNA表达明显降低 ;第 9周羟脯氨酸含量明显降低 ;组织病理观察显示绿茶提取物处理组肝纤维化程度比肝硬化组轻。结论 补充绿茶提取物能减轻CCl4 所致的肝硬化 ,可能是由于绿茶提取物的抗氧化作用导致肝组织TGF β1 mRNA表达的降低 ,从而抑制了TGF β1 介导的纤维增生、沉积过程。
Objective To study the protective effect of green tea extract on chronic liver injury using carbon tetrachloride rat liver cirrhosis model. Methods Animals were divided into 3 groups: normal control group, green tea extract treatment group, and liver cirrhosis group. The green tea extract treatment group and the liver cirrhosis group were injected subcutaneously with 0 3 ml/100 g of 40% CCl4 twice a week. Rats were sacrificed in two batches on the 2nd week and 9th week after the injection of CCl4. The hepatic hydroxyproline content, malondialdehyde (MDA) content of hepatic lipid peroxidation, pathological changes of hepatic tissue, and expression of transforming growth factor β1 (TGFβ1) mRNA in liver tissue were observed. Results Compared with the cirrhosis group, the content of MDA and the expression of TGFβ1 mRNA decreased significantly in the treatment group of the green tea extract compared with the cirrhosis group. The content of hydroxyproline was significantly decreased in the 9th week. Histopathological observation showed that the liver fiber was treated with the green tea extract treatment group. The degree of lighter than the cirrhosis group. Conclusion Supplementation with green tea extract can reduce CCl4 induced cirrhosis, which may be due to the antioxidant effect of green tea extract leading to a decrease in the expression of TGFβ1 mRNA in liver tissue, thereby inhibiting the TGF β1 -mediated fibrogenesis and deposition process.