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目的 :探讨一氧化氮体系在慢性低O2 高CO2 肺动脉高压形成中的作用。方法 :雄性Sprague -Daw ley大鼠分为正常对照组和 4周低O2 高CO2 肺动脉高压组。测定血浆NO含量 ,免疫组化法检测肺细小动脉cNOS和iNOS活性 ,原位杂交法检测其cNOSmRNA和iNOSmRNA的表达。结果 :肺动脉高压组血浆NO含量、肺细小动脉cNOS活性和cNOSmRNA表达显著低于对照组 (均P <0 .0 1) ,而iNOS活性和iNOSmRNA表达明显高于对照组 (均P <0 .0 1)。结论 :低O2 高CO2 时肺动脉NOS活性和NOSmRNA表达的改变引起的NO变化参与了肺动脉高压的形成。
Objective: To investigate the role of nitric oxide system in the formation of chronic hypoxic hypercapnia pulmonary hypertension. Methods: Male Sprague-Dawley rats were divided into normal control group and 4-week hypoxic-hypercapnic pulmonary hypertension group. The content of NO in plasma was measured. The cNOS and iNOS activities of pulmonary arterioles were detected by immunohistochemistry. The expressions of cNOS mRNA and iNOS mRNA were detected by in situ hybridization. Results: The levels of NO, the activity of cNOS and the expression of cNOS mRNA in pulmonary arterioles in pulmonary hypertension group were significantly lower than those in control group (all P <0.01), but iNOS activity and iNOS mRNA expression were significantly higher than those in control group 1). CONCLUSIONS: The changes of NO in pulmonary artery and the expression of NOS mRNA in pulmonary artery during low O2 and high CO2 are involved in the formation of pulmonary hypertension.